Characterization of acidic proteolytic enzymes from Monterey sardine (Sardinops sagax caerulea) viscera
نویسندگان
چکیده
Total enzyme activity of whole viscera, and partial characterization of acidic proteases from Monterey sardine viscera are presented. Major proteolytic activity in alkali (pH 10) and minor activity in acid (pH 3) were detected. From purified acidic proteases, six fractions with high activity were selected. One fraction (42) showed one band on SDS–PAGE and two bands on isoelectrofocusing, with pI close to 4.0 and 4.5, respectively. The optimal pH for acidic protease activity was 2.5, with high stability in the acid range and marked loss of activity at neutral and alkaline pH. The optimum temperature was 45 C, and activity was high at 10 C, whereas denaturation occurred above 55 C. Activity was inhibited by Pepstatin A but not by SBTI or EDTA. The general characteristics of these enzymes resemble those of the digestive enzymes of other fish. Because Monterey sardine is abundant in Mexico, it is a potential source for biological reagent production. # 2003 Elsevier Ltd. All rights reserved.
منابع مشابه
Isolation and characterization of trypsin from pyloric caeca of Monterey sardine Sardinops sagax caerulea.
Trypsin from pyloric caeca of Monterey sardine was purified by fractionation with ammonium sulfate, gel filtration, affinity and ionic exchange chromatography. Fraction 102, obtained from ionic exchange chromatography, generated one band in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing. The molecular mass of the isolated trypsin was 25 kDa and sh...
متن کاملEvidence that the Migration of the Northern Subpopulation of Pacific Sardine (Sardinops sagax) off the West Coast of the United States Is Age-Based
Analysis of fish movements has been an important area of study for fisheries ecology and population dynamics for decades. Pacific sardine, Sardinops sagax, along the west coast of the United States exhibit a well-defined large-scale seasonal migration. Larger and older fish are found in the northern reaches of their range during summer and contract to southerly offshore areas for spawning durin...
متن کاملDiel behavior of sardine and anchovy schools in the California Current System
Fish schools containing Pacific sardine Sardinops sagax and northern anchovy Engraulis mordax were observed in 2 regions within the California Current System in 2 years using a combination of moored and shipboard acoustics and net sampling. Schools of sardines and anchovies off the Oregon coast followed typical diel patterns consistent with antipredator behavior, dispersing at nighttime, rapidl...
متن کاملSpawning, larval abundance and growth rate of Sardinops sagax off southwestern Australia: influence of an anomalous eastern boundary current
The temporal and spatial distributions of sardine Sardinops sagax eggs and larvae off the oligotrophic southwestern coast of Australia were examined and related to gonadosomatic index, daily growth rates of larvae and regional biological oceanography. Seasonal environmental cycles were established from remotely sensed sea surface temperature and chlorophyll concentration, wind and sea surface h...
متن کاملA cold oceanographic regime with high exploitation rates in the Northeast Pacific forecasts a collapse of the sardine stock.
The oceanographic conditions in the north Pacific have shifted to a colder period, Pacific sardine (Sardinops sagax) biomass has declined precipitously in the California Current, the international sardine fishery is collapsing, and mackerel (Trachurus symmetricus and Scomber japonicus) are thriving. This situation occurred in the mid-1900s, but indices of current oceanographic conditions and th...
متن کامل